Coding

Part:BBa_K287003:Design

Designed by: Carlos Andres Sanchez   Group: iGEM09_IPOC1-Colombia   (2009-10-21)


Chloride channel protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 214
    Illegal PstI site found at 226
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 214
    Illegal PstI site found at 226
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 1321
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 214
    Illegal PstI site found at 226
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 214
    Illegal PstI site found at 226
    Illegal AgeI site found at 1198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 370
    Illegal SapI.rc site found at 418


Design Notes

The restriction sites were added by PCR with designed prymers. Restriction site at 5' Bgl II, and 3' XhoI


Source

This part comes from a Genomic Sequence from the NCBI.

References